Molecular Diagnostics refers to Laboratory medicine or laboratory test to identify a disease by analyzing the DNA or RNA or their Protein in Human. The Molecular Diagnostics era began at the time of discovery of double helical DNA structure in 1953 by Watson and crick. In the mid-1980s, southern blot transfer hybridization assay was in major part of molecular diagnostic laboratories, technical armamentarium for current an application such as detection of gene deletion in Duchenne and muscular dystrophy and repeat expansion in fragile X syndrome. Originally southern blot was primarily used in linkage analysis for cystic fibrosis and other genetic diseases. The southern blot assay became a gold standard test was used to detect many human gene sequences by the use of specific restriction endonuclease, and also used for mutation detection and gene arrangement. GenProb Inc. became the first US Company to provide the FDA approved probe-based diagnostic test for the detection...